Survival and viability of fresh and frozen - thawed in vitro bovine blastocysts A Massip P Mermillod A
نویسندگان
چکیده
horbar; Three experiments were undertaken to investigate the effect of some factors on survival and viability of bovine blastocysts produced in vitro in serum-free oviduct-conditioned media. In vitro survival to freezing was higher for blastocysts appearing on days 6 and 7 than for day 8 blastocysts (67 and 50% hatching rate vs 12%). Transfer success of day 8 blastocysts to recipient heifers was 35% (9/26) calves born vs 19% (4/21) for cows. Embryo mortality occurred mainly during the periimplantation period (between days 21 and 45) for cows compared with heifers. Calving rate after direct
منابع مشابه
Survival and viability of fresh and frozen-thawed in vitro bovine blastocysts.
Three experiments were undertaken to investigate the effect of some factors on survival and viability of bovine blastocysts produced in vitro in serum-free oviduct-conditioned media. In vitro survival to freezing was higher for blastocysts appearing on days 6 and 7 than for day 8 blastocysts (67 and 50% hatching rate vs 12%). Transfer success of day 8 blastocysts to recipient heifers was 35% (9...
متن کاملThe Analysis of Factors Affecting the Success Rate of Frozen Thawed Embryos in IVF / ICSI Cycles
Purpose: the aim of this study is to analyze the factors contributing to the success of frozen- thawed embryo transfer (ET). Material and method: This retrospective, case-control study was carried out on 700 infertile couples treated with IVF and ICSI and had embryo freezing in Isfahan Fertility and Infertility Center, Iran, from the beginning of July 2000 to the end of June 2003. Results: fa...
متن کاملImpact of in vitro fertilization by fresh and frozen semen on developmental competence and cryotolerance of buffalo embryos
Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...
متن کاملIn vitro production of bovine embryos using flow-cytometrically sexed sperm
The investigation aimed to compare the effect of fresh and frozen-thawed X and Y fractions of flowcytometrically sorted bovine spermatozoa on in vitro fertilization of bovine in vitro matured oocytes and subsequent blastocyst development. Sperm cells sorted in MoFloSX cytometer were used either for IVF or frozen and stored in liquid nitrogen. Immature oocytes recovered from ovaries of slaughter...
متن کاملEvaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal
In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...
متن کامل